Composite

Part:BBa_K1460002

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-14)
Revision as of 19:30, 14 August 2014 by Registry (Talk | contribs)

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RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter

his part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with an appropriate promoter. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 118


[edit]
Categories
//binding/metal
Parameters
None