Coding
OneProt
Part:BBa_K1475001:Design
Designed by: Camilla Kongerslev Jakobsen Group: iGEM14_SDU-Denmark (2014-08-05)
OneProt coding region
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The WHO advised amino acid composition was written down and the sequence was then shuffled using (LINK). A 3*FLAG-tag was then added by remiving the respective codons and adding them to the end of the protein to keep the right composition of amino acids.
Source
The sequence is based on WHO guidelines for amounts of amino acids needed in a normal human.