Measurement

Part:BBa_J119363

Designed by: Kelly Cochran, Mike Quaney   Group: Eckdahl Lab   (2014-07-22)
Revision as of 19:55, 22 July 2014 by Eckdahl (Talk | contribs)

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Ammeline Fitness Module with Riboswitch N2 + TetA (T85A, T89A, G93C, T94C, G95T, T96G)

The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2 with mutations T85A, T89A, G93C, T94C, G95T, T96G) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 506
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 1034
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None