Coding

Part:BBa_K1260002

Designed by: Zhengfeng Liu   Group: iGEM14_Shenzhen_SFLS   (2014-05-23)
Revision as of 18:04, 27 June 2014 by JeffLiu (Talk | contribs)

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membrane protein LGT with a SH3 domain

The gene has a membrane protein LGT and a SH3 domain. With the fusion protein it expressd on the E.coli's membrane, you can attach the proteins with a SH3 ligand on its tail.

Result

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fig.1: The Prediction Structure of the Fusion Protein. 

C. The schematic layout of SH3 domain-LGT. D. The simulated diagram of SH3 domain-LGT.
The red part is SH3 domain and the orange part is the signal peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1084
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 705
    Illegal SapI.rc site found at 202


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Categories
Parameters
None