Composite

Part:BBa_K1300008

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-19)
Revision as of 01:25, 21 June 2014 by J.reyes.97 (Talk | contribs)

recA promoter + translational GFP

The recA promoter is an inducible promoter that responds to DNA damage which can be caused by UV radiation (namely the formation of ssDNA). The purpose of this part is to test the effectiveness of the recA promoter by examining the amount of GFP formed under various UV radiation conditions.


Sequencing results compared with expected DNA sequence Recaplusgfp.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865


[edit]
Categories
Parameters
None