Composite

Part:BBa_K1300011

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-19)
Revision as of 01:21, 21 June 2014 by J.reyes.97 (Talk | contribs)

cI repressible promoter + GFP + pBAD + cI repressor

This part is used to characterize how well the pBAD part works. The pBAD promoter is an araC repressible promoter that controls for the expression of the Cubitus interruptus (CI) repressor. Without araC, CI will be produced and inhibit the pCI promoter. As a result, GFP is not produced. However, if araC is present, then the pBAD promoter will be repressed and the CI repressor will not be expressed. As a result, GFP expression will occur.


Sequencing results compared with expected DNA sequence Cipluspbad.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1065
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1005
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719


[edit]
Categories
Parameters
None