Composite
pUV + NhaS

Part:BBa_K1255002:Design

Designed by: Fernanda Puente, Marina Emilio   Group: iGEM14_CIDEB-UANL_Mexico   (2014-06-16)
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NhaS with an RFP reporter induced by an UV promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 890
    Illegal AgeI site found at 1002
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NhaS (BBa_K1255000) sequence was optimized for its expression in E. coli


Source

RFP comes from Discosoma striata (coral) and NhaS from Bacillus firms

References