Plasmid_Backbone

Part:BBa_K1256001:Design

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-05-29)
Revision as of 14:41, 9 June 2014 by F91445122 (Talk | contribs) (→‎Design Notes)


pSB1C3-Plac-NB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found at 1076
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3124
    Illegal NheI site found at 221
    Illegal SpeI site found at 1077
    Illegal PstI site found at 1091
    Illegal NotI site found at 1084
    Illegal NotI site found at 3130
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3124
    Illegal BamHI site found at 933
    Illegal XhoI site found at 2108
    Illegal XhoI site found at 3000
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3124
    Illegal suffix found at 1077
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3124
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3139
    Illegal SpeI site found at 1077
    Illegal PstI site found at 1091
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Mingdao PSB1C3-Plac-NB.png

NheI and BamHI sites were introduced just behind RBS (B0034) and in front of the terminator (B0015) on the vector of pSB1C3. This design

Source

References