Composite

Part:BBa_M36666:Design

Designed by: Ian Hull, Poorwa Godbole   Group: Stanford BIOE44 - S11   (2014-04-30)
Revision as of 21:23, 30 April 2014 by Ianhull (Talk | contribs) (→‎References)

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HeLa beta-galactosidase actuator with secretion tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 411
    Illegal BsaI site found at 1025
    Illegal BsaI.rc site found at 934


Design Notes

The lacZ alpha fragment and lacZ omega fragment were both codon optimized for expression in HeLa cells. After combining all the parts, there were only 7 repeats of ≥10 base pairs, so in order to maintain codon optimization, no further changes were made.


Source

Kozak sequence obtained from Part:BBa_J63003. Secretion tag obtained from Part:BBa_K782062. LacZ alpha fragment obtained from Part:BBa_E0051. LacZ omega fragment obtained from Part:BBa_K891003. Poly-A tail obtained from Part:BBa_M36096.

References

Moosmann P, Rusconi S. 1996. Alpha complementation of LacZ in mammalian cells. Nucleic Acids Res. 24:1171–1172.