Regulatory

Part:BBa_M45113:Design

Designed by: Federico C. Rodriguez   Group: UtahState_BE5930_-_S14   (2014-04-17)
Revision as of 08:08, 17 April 2014 by Fede crp@hotmail.com (Talk | contribs) (→‎References)


Uranium Inducible (urcA) Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2
    Illegal NgoMIV site found at 317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 630
    Illegal SapI.rc site found at 584


Design Notes

The sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be considered during the creation of the sequence as a biobrick.

Source

This part comes from Caulobacter crescentus CB15 section 318 of 359 of the complete genome, subsection 7998-8971, which may be found in Genbank at accession number AE005992.1 (Hillson et al., US Patent, Feb. 6 2008).

References

Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Heavy Metal Biosensor. US Patent Application Publication. US20110117590. Feb. 6 2008.Retrieved from: file:///C:/Users/Owner/Desktop/US20110117590.pdf