Protein_Domain

Part:BBa_M36661:Experience

Designed by: Frankie Willcox, Claudia Dennler, Meelim Lee   Group: Stanford BIOE44 - S11   (2013-10-25)
Revision as of 19:56, 6 December 2013 by Fwillcox (Talk | contribs) (→‎User Reviews)

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Applications of BBa_M36661

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Stanford Location

Plasmid Name: fur protein, DNA 2.0 Gene #: 135239, Organism: K12 E.coli, Device Type: Sensor, Box Label: BIOE 44 F13, Barcode #'s of Glycerol Stocks: 0133011789, 0113010769, 0133009689, 0133011828, 0133009694, 0133011775(This one used to Restart Stock for Fur Sensor Project)

User Reviews

UNIQ9fff6984d6300f22-partinfo-00000001-QINU ANOVA of BETA GAL Round 1—p=.239 NOT SIGNIFICANT ANOVA of BETA GAL Round 2—p=.389 NOT SIGNIFICANT

Student t-test Beta Gal round 2 -4 vs -7: t= -1.43 sdev= 6.85 degrees of freedom = 14 The probability of this result, assuming the null hypothesis, is 0.18(Not significant)


-4 vs no iron: t= -1.80 sdev= 9.15 degrees of freedom = 12 The probability of this result, assuming the null hypothesis, is 0.097( not significant)

-7 vs no iron: t=-0.823 sdev= 9.03 degrees of freedom = 12 The probability of this result, assuming the null hypothesis, is 0.43(Not significant)

Betagal1.png

Our second round of experimentation did not yield a significant difference between groups, suggesting that our construct is not functional.

BOTH BETA-GAL CHARTS HERE!

The results from this first round showed a promising difference between the iron concentration of 10^-4 M and no iron, as we expected. This led us to develop a second round of experimentation for look for statistical differences with more samples. Since there was not a significant difference between the groups, we also steered away from looking for a gradient and spent our efforts testing for functionality in the second round of experimentation.

PCR photo here Explain results

FLUORESCENCE PHOTO HERE[http://www.example.com link title]

Very faint fluorescence some potential expression (I’m not sure if you want to include this one or not…I think it might be better to show the two below showing that our plasmid was indeed taken up)


Amp plate—plasmid was taken up.


Kan plate—no growth as expected UNIQ9fff6984d6300f22-partinfo-00000002-QINU