Device

Part:BBa_K1092024

Designed by:   Group: iGEM13_Hong_Kong_CUHK   (2013-09-18)
Revision as of 11:47, 15 November 2013 by MaryZhu (Talk | contribs)

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T7-RBS-ssDsbA-PDZ Domain-Voltage Switch-RFP N terminus

This is biobrick is the counterpart of the other biobrick T7-RBS-ssDsbA-PDZ Ligand-Voltage sensor peptide-RFP C terminus (BBa_K1092023), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the RFP fragments (BBa_K1092105 & BBa_K1092106). In this Example, we demonstrate the possibility of using the voltage switch as a fast control of fluorescent signal. We made use of the biomolecular fluorescent complementation to achieve this purpose, and we worked on making the first reversible BiFC reaction in the world.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 362


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Categories
Parameters
None