Coding

Part:BBa_K1131007

Designed by: Ramya Prathuri   Group: iGEM13_Berkeley   (2013-10-28)
Revision as of 06:03, 30 October 2013 by Rprathuri (Talk | contribs)

mutant oleD 'ASP' glucosyltransferase; S. antibiotics (characterized)

This is the mutant version of oleD glucosyltransferase part BBa_K1131006. It, and its non-mutated version BBa_K1131006 were found to glucosylate indoxyl to produce indoxyl-B-glucoside, or indican. To the best of our knowledge, this is the first recombinant enzyme used to produce indican. Kaempferol is another substrate of this enzyme. Mutations identified by Thorson et. al. 2010 at P67T, S132F, and A242V were introduced to oleD generating the 'ASP' version of the protein. OleD 'ASP' is reported to have a much larger set of substrates it can glucosylate compared to the non-mutated oleD. This is a characterized version of BBa_K1131005with about 15 amino acids removed at the end that improved solubility greatly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 928
    Illegal NgoMIV site found at 1118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387
    Illegal BsaI.rc site found at 653
    Illegal BsaI.rc site found at 1078


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Categories
Parameters
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