Regulatory

Part:BBa_K1119010:Design

Designed by: Wai In Hui   Group: iGEM13_Hong_Kong_HKUST   (2013-10-24)
Revision as of 02:55, 29 October 2013 by Wihui (Talk | contribs) (→‎Source)


Human Elongation Factor-1alpha Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable

Source

The EF-1alpha promoter sequence was cloned out from plasmid iDUET101a (http://www.addgene.org/browse/sequence_vdb/2491/) using PCR with primers that includes prefix and suffix in RFC10 standard.

References

Qin, Jane Yuxia, Li Zhang, et al. "Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter." PLoS ONE. 5.5 (2010) <http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010611>.