Part:BBa_K1021017
PT7+crtB
This part is the crtB gene (the second component of the carotenoid biosynthesis pathway) downstream of the T7 promoter.
Adapted from KEGG reference pathways
crtB was originally from the 2008 Edinburgh team that isolated the coding sequence of crtB from Pantoea ananatis. crtB encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612).
The T7 promoter system was used in order to express multiple genes simultaneously. Fungal promoters are quite long, and prokaryotes have systems to splice out long repetitive sequences of DNA. Because the T7 is a short sequence, each of the carotenoid genes could be put behind the T7 promoter for maximum simultaneous transcription of the carotenoid genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 616
Illegal NgoMIV site found at 746 - 1000COMPATIBLE WITH RFC[1000]
None |