Part:BBa_K1129046

4-coumarate 3-hydroxylase under pTET constitutive promoter

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 100
  Illegal NgoMIV site found at 108
  Illegal NgoMIV site found at 858
  Illegal NgoMIV site found at 1031
  Illegal NgoMIV site found at 1433
  Illegal NgoMIV site found at 1437
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 632
  

Background

4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enzyme in the biosynthetic pathway of vanillin bioproduction from the amino acid tyrosine. 4-CMH is biobrick (BBa_K238007) from KULeuven iGEM 2009

Experimental Data

Figure 3. Compound generation identification by GC-MS. Chromatograms and mass spectra for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using caffeic acid. B) Conversion of p-coumaric acid to caffeic acid by constitutive expressed 4-CMH. C) Possible conversion of p-coumaric acid to caffeic acid by inducibly expressed 4CMH. The mass spectrum however is confounded by another compound.