Reporter

Part:BBa_K1021020

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)
Revision as of 00:43, 29 October 2013 by S.Dong (Talk | contribs)

PT7+GFP

The gene for green fluorescent protein sits downstream of the T7 constitutive promoter. This gene can be naturally expressed in Escherichia coli BL21-A1 in conjunction with T7 polymerase and can be expressed in Escherichia coli DH5α after co-transformation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


[edit]
Categories
Parameters
None