Part:BBa_K1045002

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

This part is a reporter system.
It is composed of the ydaO riboswitch from Bacillus subtilis genomic DNA with its native promoter and its native RBS (BBa_K1045004), controlling the expression of cfp as a reporter gene (BBa_E0020).

Ideally, in the presence of c-di-AMP, the riboswitch leads to premature termination of cfp transcription. In the absence of c-di-AMP, the system uses the terminator present in the pSB1C3 backbone. This results in the formation of a cfp mRNA which can be translated in a functional CFP protein. Thus, in the abscence of c-di-AMP, the system should fluoresce. In the presence of c-di-AMP, however, the system should not fluoresce.

Furthermore, in combination with the diadenylate cyclase domain (BBa_K1045003), the riboswitch reporter system could indicate the state of the c-di-AMP biosynthesis. This could be exploited in order to screen substances for effects on c-di-AMP homeostasis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]