Reporter

Part:BBa_K1088010

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-20)
Revision as of 18:17, 28 October 2013 by Thigers (Talk | contribs)

E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

This part consist of the dxs gene derived from E. coli fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS. To repress expression from the lac promoter, the lacI:LVA (gene under a constitutive promoter with a strong RBS and a efficient terminator) was placed counter-clockwise to the reporter fusion. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3937
    Illegal SapI.rc site found at 2127


[edit]
Categories
Parameters
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