Generator

Part:BBa_K1189033:Design

Designed by: Ali Honarmand   Group: iGEM13_Calgary   (2013-09-27)
Revision as of 10:20, 28 October 2013 by Cmwinter (Talk | contribs)

EHEC TALE2 (binds to a 19bp region of stx2 gene)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2404
    Illegal BamHI site found at 2539
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A KasI restriction cut site has been incorporated in the end of the TALE, so that part can easily be used to replace the TALEs in our other constructs.


Source

This part is being constructed using IDT G-Blocks.

References

Meckler, J.F., Bhakta, M.S., Kim, M.S., Ovadia, R., Habrian, C.H., Zykovich, A., ... Baldwin, E.P. (2013). Quantitative analysis of TALE-DNA interactions suggests polarity effects. Nucleic Acids Research, 41(7), 4118–28. doi:10.1093/nar/gkt085