Part:BBa_K1189033

EHEC TALE2 (binds to a 19bp region of stx2 gene)

The Calgary iGEM 2013 team sought to detect pathogenic enterohemorrhagic E. coli (EHEC) DNA using pairs of TALEs designed to pathogenic E. coli markers. This TALE was designed based on a sequence alignment of the stx2 gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including E. coli O157:H7, as well as several other EHEC strains.

Figure 1. Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.

<figure> <img src="" alt="EHEC TALE 2 SOE PCR Product" width="300" height="400"> <figcaption>

Figure 2. Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2404
  Illegal BamHI site found at 2539
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]