Coding

Part:BBa_K1104101

Designed by: Yin-Chih Chen, LIANG-CHUN CHEN   Group: iGEM13_NYMU-Taipei   (2013-10-27)
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RFP-FimH(mannose-binding domain) fusion protein

FimH protein, a protein expressed in Escherichia coli K-12 substr MG1655, has a mannose-binding site from the 22nd to 179th amino acid. FimH protein is originally located on the tip of the fimbria of the E. coli, which will adhere to the mannose on the host's cell membrane, allowing the bacteria to colonize various host tissues. In our project we managed to use it to bind the mannose polymer, which serves as a sticky end to bind the host cell, on top of the PTP1, in order to stop the adhesion between the polar filament and the epithelial cell of the bee's midgut.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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