Coding

Part:BBa_J37029:Design

Designed by: Jonathan Wells   Group: iGEM06_Imperial   (2006-08-14)
Revision as of 10:57, 14 August 2006 by Jonny (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cre recombinase, enzyme excises sequence between loxP sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Genomic sequence from enterobacteria phage P1

References

  • [http://www3.interscience.wiley.com/cgi-bin/fulltext/70001850/PDFSTART Cre recombinase: The universal reagent for genome tailoring]
  • [http://nar.oxfordjournals.org/cgi/content/full/30/17/e90 Cre recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein]