Composite

Part:BBa_K1139025

Designed by: Shinya Suzuki   Group: iGEM13_Tokyo_Tech   (2013-10-21)
Revision as of 16:01, 26 October 2013 by 382069 (Talk | contribs)

No part name specified with partinfo tag.

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter upstream of g2p is altered to bad promoter. A phage particle is formed only when the host cell receives arabinose because g2p (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.
deleted. The capsid is formed only when g2p is expressed.

Sequence and Features No part name specified with partinfo tag.


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