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Part:BBa_K1189029:Design

Designed by: Julie Osgood   Group: iGEM13_Calgary   (2013-09-17)
Revision as of 01:05, 26 October 2013 by Kate (Talk | contribs)

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TALE-A with a his tag linked to a K coil under the control of a LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1922
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2651
    Illegal AgeI site found at 677
    Illegal AgeI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

asdasd


Source

asdasd

References

  • Litowski, J. R., & Hodges, R. S. (2002). Designing heterodimeric two-stranded alpha-helical coiled-coils. Effects of hydrophobicity and alpha-helical propensity on protein folding, stability, and specificity. The Journal of Biological Chemistry, 277(40), 37272–9. doi:10.1074/jbc.M204257200