Protein_Domain

Part:BBa_M36661:Design

Designed by: Frankie Willcox, Claudia Dennler, Meelim Lee   Group: Stanford BIOE44 - S11   (2013-10-25)
Revision as of 21:02, 25 October 2013 by Fwillcox (Talk | contribs) (Created page with "Design notes: The fur protein amino acid sequence was confirmed in the RCSB Protein Data Bank [1]. Using an online DNA and Protein Sequence Optimizer tool [2], the amino acid se...")

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Design notes: The fur protein amino acid sequence was confirmed in the RCSB Protein Data Bank [1]. Using an online DNA and Protein Sequence Optimizer tool [2], the amino acid sequence to a nucleotide sequence specific for K12 E. coli codons, using the favored codon 70.3% of the time. This optimization yielded a 52% GC content and a 48% AT content. In addition, a His-tag, used by Woestenek [3], which will be used as the primary antibody binding site if we need to conduct a Western blot assay. The fur protein was modified with a stop codon following the His-tag.