Tag

Part:BBa_K1159313

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-17)
Revision as of 15:09, 23 October 2013 by AndiB (Talk | contribs) (→‎Usage and Biology)

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N-terminal His6-tag in RFC[25] N-Part

This parts codes for a N-terminal His6-tag flanked by RFC[25] N-part to allow C-terminal fusions


Usage and Biology

The so called N-terminal encoded 6xHis-Tag consists of six histidine residues and allows the C-terminal fusion proteins for protein purification after protein expression in E. coli. The iGEM Team of TU Munich 2013 used the 6x His-Tag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None