Coding

Part:BBa_K1031311

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-10-13)
Revision as of 07:44, 14 October 2013 by Psyche (Talk | contribs)

J23114-HbpR-Terminator

For detailed information concerning HbpR, please visit 2013 Peking iGEM Biosensor HbpR


Characterization

In order to fine-tune the performance of HbpR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031311 is composed of three elements, the constitutive Pc promoter J23114, coding sequence of HbpR and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23114-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 449


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//function/regulation/transcriptional
//terminator/double
Parameters
positive_regulators