Measurement

Part:BBa_K1055000:Experience

Designed by: Kai Fenzl   Group: iGEM13_TU_Darmstadt   (2013-09-18)
Revision as of 18:20, 12 October 2013 by Arne (Talk | contribs) (→‎Applications of BBa_K1055001)

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Applications of BBa_K1055001

When tried to fuse to the CheB signaling protein of E. coli C-terminally, we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 232 kj/mol). Please take a look below:

Hypothetical DNA secondary structure of CheB-mKate fusion protein which might be the reason for continously failing clonation attempts. We derived dG = - 232 kj/mol

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