Measurement

Part:BBa_K1055001:Experience

Designed by: Kai Fenzl   Group: iGEM13_TU_Darmstadt   (2013-09-18)
Revision as of 18:09, 12 October 2013 by Arne (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1055001

When tried to fuse to the Tar receptor of E. coli C-terminally we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 318 kj/mol). Please take a look below:

Hypothetical DNA secondary structure of Tar-LssmOrange fusion protein which might be the reason for continously failing clonation attempts. We derived dG = - 318 kj/mol

User Reviews

UNIQa02a17a2ac245cb7-partinfo-00000000-QINU UNIQa02a17a2ac245cb7-partinfo-00000001-QINU