Coding

Part:BBa_K1071002

Designed by: Team Marburg 2013   Group: iGEM13_Marburg   (2013-09-19)
Revision as of 18:05, 12 October 2013 by Lucas (Talk | contribs)

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cat resistance (procaryotic)

Chloramphenicol interacts with the 50s Subunit of bacterial ribosomes and blocks peptidyltransferase activity. The antibiotic is acting on gram negative as well as on gram positive bacteria. The enzyme Chloramphenicol acetyltransferase (CAT) transfers one or two Acetyl groups to the hydroxyl-Residues of Chloramphenicol. This inactivates the antibiotic.


Usage and Biology

This chloramphenicol resistance gene was used in our expression vector to cultivate the plasmid in E. coli. We successfully selected the colonies on LB plates with chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None