Reporter

Part:BBa_K1071009

Designed by: Team Marburg 2013   Group: iGEM13_Marburg   (2013-09-19)
Revision as of 17:55, 12 October 2013 by Lucas (Talk | contribs)

eGFP

the fluorescent protein eGFP, codon usage of E. coli, works also in Phaeodactylum tricornutum. The excitation peak is at 488nm and the emission at 507nm.

Usage and Biology

In the following picture we demostrate that both promotors (i.e. PNR and PfcpB) work properly as shown by the expression of eGFP (green, second panel). The red color (third panel) is due to autoflourescence of the plastides and indicates their localisation within P. tricornutum. The very left and the very right panel represent DIC and an overlay of green and red, respectively.

eGFP in P. tricornutum

eGFP localisation in P. tricornutum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None