Coding

Part:BBa_K1223013

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-10-03)
Revision as of 13:58, 12 October 2013 by Alex m (Talk | contribs)

Pyrolysyl-tRNA synthetase CDS

this part is the coding sequence for the Pyrolysyl-tRNA synthetase enzyme from the archaea Methanosarcina barkeri str. fusaro. The enzyme 'load' the amino acid pyrolysine onto its dedicated tRNA for subsequent incorporation into proteins during the translation process. The tRNA synthetase is part of the unnatural amino acid (UAA) incorporation machinery along with the dedicated tRNA molecule (BBa_K1223014).

Design Notes

CDS of the pyrolysyl-tRNA synthetase (pylS) that charges the pyrolysine tRNA (pylT) with pyrrolysine.

Source

derived from the genomic sequence of Methanosarcina barkeri. gene name - pylS

clasification: Archaea : Euryarchaeota : Methanomicrobia : Methanosarcinales : Methanosarcinaceae : Methanosarcina

References

Srinivasan,G., James,C.M. and Krzycki,J.A., Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA, Science 296 (5572), 1459-1462 (2002)

Milles, S.; Tyagi, S.; Banterle, N.; Koehler, C.; VanDelinder, V.; Plass, T.; Neal, A. P.; Lemke, E. A., Journal of the American Chemical Society 2012, 134 (11), 5187-95.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 394
    Illegal BglII site found at 755
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90
    Illegal BsaI.rc site found at 128


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Categories
Parameters
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