Plasmid_Backbone

Part:BBa_K1223009

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-09)
Revision as of 13:39, 12 October 2013 by Alex m (Talk | contribs)

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pUC57 backbone (REVERSED)

pUC57 backbone - digested between BamHI and XbaI. (reverse-complement sequence)

this part is the backbone of the widely used high copy number plasmid pUC57. this plasmid is used to overexpress recombinant proteins as well as amplify a gene of interest inside E.coli. the plasmid backbone contains ampicillin resistance gene (b-lactamase) for selection of transformants.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1341
    Illegal SapI.rc site found at 2423


[edit]
Categories
Parameters
None