Coding

Part:BBa_K1152009

Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis   Group: iGEM13_Heidelberg   (2013-09-19)
Revision as of 22:58, 5 October 2013 by Herbstk (Talk | contribs)

Sfp 4'-Phosphopanthetheinyl-transferase

Sfp encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes.

  • Figure 1: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

We hereby improve parts BBa_K802006 and BBa_K302010 which also encode for sfp from Bacillus subtilis. Both parts are equal in sequence dispite the fact that obviesly BBa_K802006 includes additional bases upstream from the B. subtilis genome. we improved these parts in availablity to the community as well as in characterization.

By MSA we can conclude that BBa_K1152009 is different from BBa_K802006 and BBa_K302010 at 3 different amino acid sites: File:Heidelberg2013 BBa sfp comparement.clustal.txt.

We can also conclude that BBa_K802006 is actually sfp even when not annotated like this.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal SapI.rc site found at 661


[edit]
Categories
Parameters
None