Coding

Part:BBa_K1211001:Experience

Designed by: Joel Sher   Group: iGEM13_Yale   (2013-08-16)
Revision as of 22:55, 5 October 2013 by Jws67 (Talk | contribs) (→‎Applications of BBa_K1211001)


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Applications of BBa_K1211001

This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA.

We (Yale iGEM 2013) used this enzyme as a the first enzyme in the pathway to create PLA or Polylactic acid. However, this biobrick produces (D)-Lactyl-CoA, which is the precursor to all PHAs, and thus this biobrick can be used in a wide range of projects in the future.

User Reviews

UNIQ9e98f8e425a3cd53-partinfo-00000000-QINU UNIQ9e98f8e425a3cd53-partinfo-00000001-QINU