![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1211001:Design
Designed by: Joel Sher Group: iGEM13_Yale (2013-08-16)
Clostridum propionicum propionate CoA transferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA. The mutations were chosen based upon Yang et al. 2009.
Source
The sequence of the wild type enzyme came from GenBank.