Coding

Part:BBa_K1211001:Design

Designed by: Joel Sher   Group: iGEM13_Yale   (2013-08-16)
Revision as of 22:54, 5 October 2013 by Jws67 (Talk | contribs) (→‎Design Notes)


Clostridum propionicum propionate CoA transferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum.  The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA.  This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA. The mutations were chosen based upon Yang et al. 2009.

Source

The sequence of the wild type enzyme came from GenBank.

References