Part:BBa_K1140006:Experience
Applications of BBa_K1140006
The team observed that this part works correctly in E. coli K12.
Surprisingly, we obtained different behaviors in clones transformed with the same DNA (figure 3). All measurements were performed at least in triplicate, the aritmethic mean is shown. Figure 2 shows the behavior of our best clone, dubbed M1. M12 clone, showing a weird behavior, is to be sequenced to verify if this outcome is due to mutation or intrinsic cellular noise.
Mathematically, we found that a simple gaussian function fits our data well, and it provides us a way to quantify the strength (amplitude), optimal value (horizontal shift), and definition or clearness (width) of our RNAT activity (figure 4). We believe positive slope is due to RNAT melting, while negative slope is due to increase in the overall protein degradation rate due to higher temperatures. This function also allows for comparisons between different RNAT, as well as being potentially predictive for non verified temperatures.
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