Composite

Part:BBa_K1144015

Designed by: Laura Rodriguez   Group: iGEM13_Colombia_Uniandes   (2013-09-18)
Revision as of 22:09, 5 October 2013 by Sylvita1015 (Talk | contribs)

Prcna, hoxN


Prcnr is a constitutive promoter, controlling the production of RcnR protein which

represses PrcnA. When there is nickel in the medium RcnR this induces a conformational

change in RcnR which prevents its binding to PrcnA, activating the production of the HoxN

protein in our construct. HoxN an efficient nickel membrane transporter form Cupriavidus

metallidurans that imports the metal into the cell. RFP was transcriptionally fused to HoxN

to monitor its expression.

Therefore, our system continuously detecting for nickel, and when this metal is detected,

it is absorbed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 945
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Eitinger, T. Microbial Nickel Transport In Microbial Transport Systems. Ed. G. Winkelmann. Wiley-VCH 2001

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