Coding

Part:BBa_K1190000:Design

Designed by: Nisarg Patel   Group: iGEM13_Arizona_State   (2013-08-30)
Revision as of 10:20, 5 October 2013 by Napatel (Talk | contribs) (→‎Design Notes)

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Melan-A (MART-1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural Melan-A sequence has a PstI site at 323bp. This can be remedied by creating a silent mutation to get rid of the PstI site. Because the epitope site is from amino acids 27-35 (81-105bp), it is also possible to shorten the sequence to less than 323 base pairs to exclude the PstI site altogether while retaining protein function as a vaccine candidate.


MelA ov-seq This part has been sequence confirmed, quantitatively demonstrating correct identity of the biobrick.

Source

Homo sapiens melanocytes

References