Coding

Part:BBa_K1104300:Design

Designed by: Jou-Chien Liao, Rohan Shina, Yucheng Huang   Group: iGEM13_NYMU-Taipei   (2013-09-11)
Revision as of 08:02, 5 October 2013 by JRDeath (Talk | contribs) (→‎Design Notes)

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Abaecin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Abaecin primers(example)

mature peptide
ATGTATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCTATTAA (108nt)
ATGTATGTACCATTACCAAATGTACCAC (36nt)
    ATGTACCATTACCAAATGTACCACAACCAGGACGTCGTCCGTTTCCAACGTTTCCTGGT (59nt)
                                            GTTTCCAACGTTTCCTGGTCAGGGACCATTCAATCCGAAAATTAAATGGCCTCAAGGCT (59nt)
                                                                                   AATTAAATGGCCTCAAGGCTATTAA tactagtagcggccgctgcagtcc (49nt)
           24nt,55C                               19nt,54C                                20nt,54C
  • See more:[ http://www.ncbi.nlm.nih.gov/nuccore/NM_001011617.1]、[ http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=406144]

Source

Apis melliferra

References