Regulatory
Part:BBa_K950002
Designed by: Jakob Matthes Group: iGEM12_Tuebingen (2012-07-29)
Revision as of 03:48, 5 October 2013 by Jakobmatthes (Talk | contribs)
yeast anb1 promotor
This is the promotor of yeast ANB1 gene. Panb1 is a target of the rox1 repressor, see Part BBa_K950001
Increased promoter activity of Psuc2 and Panb1 in repressor deficient strains. Fluorescence was detected using a RFP filter set (ET Bandpass 470/40, ET Bandpass 572/35).
Promoter activity of Psuc2 and Panb1 in repressor deficient strains. The promoter activity was enhanced by a factor of 2.6 for Psuc2 and 13.8 for Panb1. Fluorescence was measured using a plate reader (Ex: 548nm +/-9nm, Em: 581nm +/-20nm) and normalized to OD600 of the cell suspension. All measurements were performed as triplicates.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 124
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/yeast
//promoter
//regulation/negative
//promoter
//regulation/negative
None |