Composite

Part:BBa_K1132034

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-19)
Revision as of 03:41, 5 October 2013 by Diox (Talk | contribs)

AND gate (BBa_K1132003) with RFP

This BioBrick is designed to test the AND gate (BBa_K1132003) by measuring the level of RFP after recombination events. The biobrick BBa_K081014 containing the RBS site, the coding sequence of the RFP has been inserted inside our gate between the FimE restrictions sites. However, to detect expression of RFP, the T7 polymerase is required. An amelioration of this BioBrick has been created, adding a strong promoter, an RBS and the open reading frame of the T7 polymerase to the construct (BBa_K1132037).

2034.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 957
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 264
    Illegal AgeI site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


See BBa_K1132037 for characterization.

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Categories
Parameters
None