Composite

Part:BBa_K1132031:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-19)
Revision as of 03:19, 5 October 2013 by Diox (Talk | contribs) (→‎Source)

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Test of the AND gate (BBa_K1132001) with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 286
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 295
    Illegal AgeI site found at 1091
    Illegal AgeI site found at 1203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 315
    Illegal BsaI.rc site found at 476


Design Notes

This BioBrick is design to test the AND gate (BBa_K1132001) by measuring the level of RFP after recombination events. The biobrick Bba_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been assembled with our gate.

2031.png

Source

Cloning assembly.

References

[http://www.ncbi.nlm.nih.gov/pubmed/23539178 Amplifying genetic logic gates. Bonnet et al.]
[http://www.ncbi.nlm.nih.gov/pubmed/23396014 Synthetic circuits integrating logic and memory in living cells. Siuti et al.]