Composite
Part:BBa_K1132036:Design
Designed by: iGEM Toulouse Group: iGEM13_INSA_Toulouse (2013-09-19)
XOR gate (BBa_K1132004) with RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 178
Illegal AgeI site found at 290 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This BioBrick is design to test the XOR gate (BBa_K1132004) by measuring the level of RFP after recombination events. The biobrick Bba_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been inserted inside our gate between the FimE restrictions sites.
Source
Cloning assembly.
References
[http://www.ncbi.nlm.nih.gov/pubmed/23539178 Amplifying genetic logic gates. Bonnet et al.]
[http://www.ncbi.nlm.nih.gov/pubmed/23396014 Synthetic circuits integrating logic and memory in living cells. Siuti et al.]