Device

Part:BBa_K1150043

Designed by: Ilona Blank   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 02:33, 5 October 2013 by Ilona (Talk | contribs)

uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3

pH1:tracrRNA pU6:DR_dummy_DR
Function two small RNAs giving rise to

specific binding of dCas9 to two VEGF loci

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes
Source Feng Zhang, Addgene
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]



Usage and Biology

By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the [http://2013.igem.org/Team:Freiburg/Project/effector dCas9 derivates] at once. The basis of the device is the RNAimer.
Here, we combined BBa_K1150037 and BBa_K1150038.


Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
    Illegal BglII site found at 1061
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216
    Illegal BsaI.rc site found at 1053


[edit]
Categories
Parameters
None