Composite

Part:BBa_K1075049

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)
Revision as of 02:05, 5 October 2013 by CLS (Talk | contribs)

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AraC-pBAD-RBS32-sspB-TT-pLac-RBS34-mCherry-TT

This part contains sspB under the control of the AraC promoter and mCherry under the control of the pLac promoter.

Usage and Biology

The sspB protein is an adaptor responsible for delivering ssrA-tagged substrates to the ClpXP protease in order to enhance their degradation.

mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the Emission maximum at 610 nm. [http://www.ncbi.nlm.nih.gov/pubmed/15558047]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1234
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1069
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1051


[edit]
Categories
Parameters
None