Generator

Part:BBa_K1060011:Design

Designed by: Ingmar Claes, Lukas Marcelis, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-19)
Revision as of 01:43, 5 October 2013 by SanderW (Talk | contribs) (→‎Source)

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Beta Farnesene production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1409
    Illegal BamHI site found at 709
    Illegal XhoI site found at 671
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 281


Design Notes

no EcoRI, XbaI, NotI, SpeI or PstI sites present


Source

EBF synthase from Artemisia annua genome (Picaud et al., 2005; BBa_K1060002), Lac operator (Gilbert & Maxam, 1973)

References