Part:BBa_K1111005

Constitutive promoter inducing GFP expression
This plasmid contains the constitutive promoter BBa_J23119, that was designed by the 2007 Berkley Team. We inserted it into the biobrick BBa_I746908 instead of the AraC promoter. The cells that contained this construct turned bright green. We used them as a positive control to characterize two of our other parts which contain the same plasmid but have inducible pormoters instead.

Figure 1: The Plasmid containing the constitutive promoter and superfolded GFP

Usage and Biology

This plasmid had many applications, expecially in our igem project: 1.) it is used as a positive control for any promoter that is to be tested 2.) We used it for our synthetic biology Kit that was part of our Human Practice. As the fluorescence is seen by the naked eye, it makes a very nice experiments to make students interested in synthetic biology. 3.) the plasmid itself confers a chloramphenicol to the bacteria.

Figure2: Transformed bacteria from our human practice Kit.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 74
  

Functional Parameters

n/a	constitutive promoter inducing GFP expression